Applied Biosystems Real-Time PCR
PCR 혁신의 선두주자로서, Applied Biosystems는 real-time PCR 장비의 골드 스탠다드로 자리잡았습니다.
Applied Biosystems 사의 real-time PCR 장비와 TaqMan Assays, 다양한 시약 제품 포트폴리오는 real-time PCR 실험을 쉽게 시작할 수 있으면서도 우수한 성능, 신뢰성, 세계적인 지원을 통해 진정한 가치를 얻을 수 있습니다.
사용자에게 적합한 QuantStudio Real-Time 시스템을 선택하는 것은 샘플 수, 애플리케이션 및 유연성에 따라 다양하며, Applied Biosystems의 플랫폼은 OpenArray 플레이트를 이용한 대용량 처리 형식에 이르까지 사용자의 모든 요구사항을 지원합니다.
정밀한 정량 분석 및 민감도(Precision & Sensitivity)
높은 민감도 : 최소 1copy의 작은 유전자 발현 변화까지 감지하여, 미세한 차이도 놓치지 않고 분석할 수 있습니다.
넓은 동적 범위 : 최대 10log 범위의 샘플 볼륨에 대해 재현성 있는 분리 및 정량화가 가능합니다.
사용자 편의성 및 효율성(Ease of use & Efficiency)
직관적인 터치 스크린을 통해 사용이 간편합니다.
VeriFlex Thermal Block의 독립적으로 조절이 가능한 블록을 제공하여 다양한 온도 조건의 실험을 동시에 진행할 수 있습니다.
96well, 384well 등 다양한 형식의 블록을 교체 사용이 가능합니다.
연겨설 및 데이터 관리(Connectivity & Data Management)
클라우드 기반 Connect 플랫폼을 통해 진행 상황을 원격으로 모니터링이 가능하며, 데이터의 관리가 안전하고 용이합니다.
| For when you need: | Intuitive and easy to use for all levels of experience | Smarter productivity and improved workflow | Maximum productivity with minimum effort | |||
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| Ultimate simplicity | Ultimate simplicity | Total control | Smart and connected | Smarter productivity | Maximum productivity | |
| Instrument specifications | QuantStudio™ 1 System | QuantStudio™ 3 System | QuantStudio™ 5 System | QuantStudio™ 6 Pro System | QuantStudio™ 7 Pro System | QuantStudio™ 12K Flex System |
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| Colors | 3 colors | 4 colors | 5 or 6 colors (21 filter combinations) |
5 colors | 6 colors (21 filter combinations) |
6 colors (21 filter combinations) |
| Available formats* / Reaction Volume |
96-well (0.2 mL block ; 10-100ul) |
96-well (0.1 mL block ; 10-30ul)96-well (0.2 mL block ; 10-100ul) |
96-well (0.1 mL block ; 10-30ul)96-well (0.2 mL block ; 10-100ul)384-well (5-20ul) |
96-well (0.1 mL block ; 10-30ul)96-well (0.2 mL block ; 10-100ul)384-well (5-20ul)TaqMan Array Card (384-well ; ~1ul) |
96-well (0.1 mL block ; 15-30ul)96-well (0.2 mL block ; 10-100ul)384-well (5-20ul)TaqMan Array Card (384-well ; ~1ul)OpenArray plates (3,072 through-holes ; 33nl) |
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| Dimensions (H x W x D) | 40 x 27 x 50 cm | 40 x 27 x 50 cm | 40 x 27 x 50 cm | 54.7 x 33.8 x 52.5 cm | 54.7 x 33.8 x 52.5 cm | 73.8 x 50.1 x 66.1 cm |
| Excitation source | Bright white LED | Enhanced OptiFlex system, white LED |
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| Optical detection | 3 coupled filters | 4 coupled filters | 96-well: 6 decoupled filters384-well: 5 coupled filters |
5 coupled filters | 6 decoupled filters (21 filter combos) |
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| Excitation/emission range | 450–600 nm/ 500–640 nm |
450–600 nm/ 500–640 nm |
96-well: 450–680 nm/ 500–730 nm384-well: 450–650 nm/ 500–700 nm |
450–680 nm/500–730 nm | N/A | |
| Multiplexing | Up to 3 targets | Up to 4 targets | 96-well: up to 6 targets96-well: up to 6 targets | 5 targets | 6 targets | • Decoupled: 6 emission, 6 excitation (96-well/Fast, 384-well, TaqMan blocks)• Coupled: 4 emission, 4 excitation (OpenArray blocks) |
| Chemistry capabilities | Standard | Fast/standard | Fast/standard | N/A | N/A | N/A |
| Run time | ~40 min runs | <30 min runs | 96-well block: <30 min runs384-well block: <35 min runs |
<30 min runs | <30 min runs | TaqmanArray card - 30 min expected (0.1mL 96-well block) - 35 min (384-well block, using Fast master mix)Openarray block - 2 hr (gene expression) - 4 hr (genotyping) |
| Block change | Fixed | Fixed | Fixed | Interchangeable | Interchangeable | Interchangeable |
| VeriFlex temperature control | No | Yes, 3 zones | Yes, 6 zones (96-well blocks only) |
Yes, 3 zones (96-well blocks only) |
Yes, 6 zones (96-well blocks only) |
N/A |
| Throughput | Medium | Medium | Medium | High | High | Very High |
| 21 CFR Part 11–enabled | No | Security | Security, auditing, e-signature package |
Upgradeable | Security, auditing, e-signature package |
Optional security, auditing, e-signature package |
| Touchscreen | Yes, interactive (8.5 in) |
Yes, interactive (8.4 in) |
Yes, interactive (8.4 in) |
Yes, interactive (12 in) |
Yes, interactive (12 in) |
Yes, interactive (10.4 in) |
| Cloud-enabled | No | Yes | Yes | Yes | Yes | No |
| Automation compatible | N/A | N/A | N/A | Upgradeable | Yes | Yes |
| Dye compatibility | FAM/SYBR Green VIC/JOE/HEX/TET, JUN/ROX/Texas Red, /SYTO 9 |
FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN/ROX/Texas Red |
FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN/ROX/Texas Red, Mustang Purple™/ Cy5/LIZ Cy5.5 |
FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN/ROX/Texas Red, Mustang Purple/Cy5/LIZ, Cy5.5 |
SYBR, NED, Texas Red, Cy3, TAMRA, ROX, Cy5, JOE, FAM, VIC, Mustang Purple dyes※ Instrument may be calibrated within the confines of the excitation and emission filters. See available calibration plates. |
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| Key applications | • Gene expression• miRNA profiling• SNP genotyping• Copy number variation• Protein thermal shift• High resolution melt• Pathogen detection | • Gene expression• miRNA profiling• SNP genotyping• Copy number variation• Protein thermal shift• High resolution melt• Pathogen detection | • Gene expression• miRNA profiling• SNP genotyping• Copy number variation• Protein thermal shift• High resolution melt• Pathogen detection | • Gene expression• miRNA profiling• SNP genotyping• Copy number variation• Protein thermal shift• High resolution melt• Pathogen detection• Pharmacogenomics | • Gene expression• miRNA profiling• SNP genotyping• Copy number variation• Protein thermal shift• High resolution melt• Pathogen detection• Pharmacogenomics | • Gene expression• miRNA profiling• SNP genotyping• Copy number variation• Protein thermal shift• High resolution melt• Pathogen detection• Pharmacogenomics• Growing menu of qualified solutions |